Request Information
PhytoGEM - Plant DNA extraction
PhytoGEM - Plant DNA extraction

The PhytoGEM is a rapid, robust DNA extraction device for plant based DNA which can extract DNA in under 15 minutes. The compact size means it can easily be placed in a laminar flow hood, on the benchtop or taken directly into a field environment

2 versions are available the PhytoGEM and PhytoGEMO MINI.  

The PhytoGEM PDQex2400 is a 24 sample extractor, mains operated, with a small footprint (200 x 200 mm).  Whereas the PhytoGEM MINI is a 16 sample extractor that can be operated on battery or mains power.

Extraction of DNA from plant samples by current methods is laborious and difficult. ZyGEM has developed a simple technique for collecting cells from leaves, seeds, roots, stems and cotyledons while providing a storage option and generating PCR-ready DNA.  The cell collection takes less than a minute. Using ZyGEM’s PDQeX technology for DNA extraction, total processing time is approximately 15 minutes from a plant sample to PCR-ready DNA. The phytoGEM collection cards allow for plant samples to be stored at room temperature for months at a time, removing the need for freezing of samples and cold storage. As the PDQeX machine is small and lightweight, collection and extraction can be done in the greenhouse with no need to take samples back to the lab.

Extracting DNA from plants can be challenging because of their tough cell walls and the inhibitory polysaccharides and tannins released during the extraction process. Currently available methods address these difficulties by using tissue grinding followed by silica beads, columns or organic solvents. These procedures are multistep making them labour intensive and time-consuming.

The phytoGEM extraction system is a simple closed-tube method for extracting PCR-ready plant DNA. It uses a collection tool that crushes the material onto storages cards. Punches from the card are lysed using an aggressive thermophilic proteinase combined with a cocktail of cell wall degrading glycosyl hydrolases. The extraction is performed automatically in the ZyGEM PDQeX2400 device which both lyses the cells and purifies the DNA through a proprietary polymer matrix.

All-up, it takes around 15 minutes to go from leaf to DNA … And most of that time is hands-off.

To demonstrate the extraction efficiency, plant DNA extraction system, six plants were selected: Arabidopsis thaliana, Oryza sativa (Rice), Zea mays (Maize), Triticum aestivum (Wheat), Solanum lycopersicum (Tomato) and Citrus limon (Lemon). The results from qPCRs and end-point PCRs are shown below.

qPCR plots of alcohol dehydrogenase (adh) gene fragments amplified from phytoGEM extractions from 6 plants.

Agarose gel images showing amplicons generated from plant extractions. Fragments were generated using primer combinations targeting sequences in 5 genes: adh (alcohol dehydrogenase), act2 (actin 2), matk (maturase K), rbcL (RuBisCO large subunit) and psbA (photosystem II) ranging from ~200–2300 bp.

  • (A)  Arabidopis: 1-4: act2, 5-6: matK, 7-8: rbcL, 9-10 psbA, 11-12: adh
  • (B)  Rice: 1-2: rbcL, 3-4: psb, 5-14: various fragments of adh.
  • (C) 1-2: lemon adh, 3-4: lemon rbcL, 5: lemon psb, 6-7: maize adh, 8-9: maize psb, 10-11: maize rbcL, 12-13: tomato psb, 14-15:  tomato rbcL.

ZyGEM phytoGEM video

 

 

Extractor Tube                

  The disposable extractor tube contains all the equipment for processing the sample and ejecting the DNA.

 

       • Eliminates transfer steps

       • Makes setup easy

       • Reduces waste

       • Protected from contamination

 

 

 

 

View / Download the datasheet

View / Download the application note

View / download the instructions

 

 

 

 

 

 

 

 

 
Add to cart