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prepGEM Universal - DNA extraction kit
prepGEM Universal - DNA extraction kit
 

Temperature-controlled, hands-free, single-tube processing makes prepGEM Universal perfectly suited for extracting DNA from cell culture, even single cells, and ensures the integrity of the sample with no loss of DNA.

prepGEM is an excellent tool for researchers performing confirmatory genotyping of transfected cells following CRISPR/Cas9 gene editing.

Depending on your laboratory equipment and end-use application, MicroGEM offers two options:

1. Using Your Lab Equipment

MicroGEM's prepGEM Universal Kit provides users with a single tube solution for extracting DNA from a variety of human samples, including liquids, buccal swabs, and blood.

For tissue culture, prepGEM Universal gives linear results from 200,000 cells down to 1 single cell. The beauty of the method is that it can be scaled down to sub-µl volumes making it perfect for single-cell work.

The kit produces single-stranded DNA which can be used for STR, PCR, WGA and qPCR.

The single-step, closed-tube lysis and extraction are highly desirable because MicroGEM extraction buffers are compatible with downstream reactions.

 

Effortless DNA extraction
 
prepGEM Universal utilizes MicroGEM’s thermophilic proteinase for simple, rapid DNA extraction with unprecedented recovery.
Sample types: cell culture, saliva, tissue, blood, and insects
 
Key advantages:
 
  • Single-tube extraction
  • Protocols ranging from 4 to 18 minutes
  • High DNA Recovery – Minimal loss of DNA during extraction
  • No magnetic beads – No spin-columns – No harsh chemicals
  • Protocols scalable from 1 cell to millions of cells
  • Easily automated on a standard laboratory thermocycler or robotic platform
  • Minimal plasticware required – Reduced waste
 
This kit is an excellent tool for researchers performing confirmatory genotyping of transfected cells following CRISPR/Cas9 gene editing.
 
Each kit contains: prepGEM, Histosolv, BLUE Buffer, RED+ Buffer, ORANGE+ Buffer

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

View / Download the flyer

View / download the quick start guide

View / Download ordering guide

Application note: CRISPR

Application note: Fast and efficient DNA extractions for CRISPR

2. MicroGEM's PDQeX Nucleic Acid Extractor

The PDQeX Nucleic Acid Extractor simplifies molecular biology workflows.

The PDQeX is best suited for larger sample sizes (>200,000 cells) when double-stranded DNA is needed, for example for NGS (Next Gen Sequencing) or other methods requiring dsDNA. The system provides greater automation and generates purified DNA that also works perfectly for STR, PCR and qPCR.

View / Download the brochure

View / Download ordering guide

 

 
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