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RNAGEM is a whole nucleic acid extraction kit providing an extremely simple method to extract RNA from a single cell to 100,000 cells. The kit includes RNase-free enzymes, buffers, and DNase I and is ideal for processing:
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mammalian cell culture
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laser capture micro-dissections (LCM)
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Fluorescence-activated cell sorting (FACS)-prepared cell populations
RT-PCR and RT-qPCR reagents can be added directly to the lysate for a simple, streamlined protocol in 96 well plates.
RNAGEM – effortless, simultaneous, total nucleic acid extraction… in minutes not hours.
RNAGEM utilizes MicroGEM’s novel thermophilic proteinase and buffer system to extract total nucleic acids in less than 25 minutes without the need for purification.
Sample Types: solid tissue, cell culture, single-cell & exosomes
Downstream applications: PCR, RT-PCR, RT-qPCR, LAMP & RT-LAMP, Nanostring
Key advantages:
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Single-Tube Nucleic acid extraction in less than 25 minutes
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Simultaneous Total Nucleic Acid Extraction – genomic DNA, mRNA, long non-coding RNA, small non-coding RNA
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No ionic detergents or chaotropic salts
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High nucleic acid recovery - Minimal loss of nucleic acids during extraction
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No magnetic beads – No spin-columns
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Flexibility - suitable for low-throughput to high-throughput extraction with a single protocol
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Easily automated using standard liquid handling solutions
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Minimal plasticware required - Reduced waste and supply chain issues
RNAGEM is for research use only (RUO), not indended for in-vitro diagnostic (IVD) use.
Each kit contains: RNAGEM, BLUE Buffer, DNase I, DNase Buffer, TE Buffer
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