Application note

Price range: £393.00 through £3,488.00 + VAT
Transform the complex challenge of sperm cell lysis into a fast, simple, and highly efficient process. forensicGEM Sperm is a specialized enzymatic kit designed for the rapid, single-tube extraction of high-quality DNA from sperm cells, semen samples, and vaginal swabs. Eliminate the need for harsh, inhibitory chemicals and lengthy purification to achieve superior yields and STR-ready DNA in just 11 minutes.
This kit delivers reliable DNA extraction from sperm cells, a cell type notoriously difficult to lyse without compromising downstream analysis. It enables efficient differential extraction workflows and y-screening, even from samples with very few sperm present. The method produces double-stranded DNA, and STR profiling, PCR, and qPCR all require dsDNA
Traditional methods rely on STR-inhibiting chemicals like DTT, SDS, and mercaptoethanol, which are toxic to downstream processes and require time-consuming purification steps that cause DNA loss. forensicGEM Sperm uses a novel, temperature-controlled cocktail of thermophilic and mesophilic enzymes.
The entire hands-off process is completed in a single tube in approximately 11 minutes. This closed-tube system eliminates transfer steps, minimizing opportunities for contamination, cross-talk, and human error while protecting sample integrity. The protocol is compatible with existing thermal cyclers and easily automated for high-throughput processing, from low-volume casework to large-scale batches. This rapid processing means sex crime cases can be triaged very quickly to determine if a Y chromosome is present in the sample.
Choose forensicGEM Sperm. It is the precise, gentle, and efficient solution for modern forensic differential extraction, accelerating justice in time-sensitive investigations.

forensicGEM Sperm DNA extraction kit