ForensicGEM Sperm DNA Extraction Kit

forensicGEM Sperm is a rapid kit for extracting DNA from tough sperm heads. Using temperature controls, this kit requires no reducing agents, such as SDS, mercaptoethanol or DTT, that inhibit qPCR and STR analysis, As a result, DNA yields are maintained, processing time is quick, and opportunities for contamination are minimized making this the perfect approach for differential extractions and y-screening. This kit works with:

• Sperm cells
• Semen
• Semen stains
• Vaginal swabs

Exymes ForensicGEM Sperm kit produces single stranded DNA validated for use with STR, PCR and qPCR.  It can extract DNA from liquid samples, dried samples and stains.

Use your lab’s existing thermocycler or liquid handler, choose the forensicGEM Sperm Kit, optimized with our proprietary enzyme cocktail to rapidly degrade sperm heads and digest the remaining tissue and nucleases.

The kit produces single-stranded DNA suitable for STR, PCR, qPCR.

Effortless, DNA extraction from sexual assault samples forensicGEM Sperm utilizes Exymes novel thermophilic proteinase and Acrosolv reagent to lyse sperm without the use of qPCR and STR inhibiting chemicals, such as SDS, mercaptoethanol and DTT. Sample types: semen swabs, stains & liquid semen

Downstream applications: STR, PCR, qPCR

Key advantages:

• Single-tube DNA extraction in 11 minutes
• No need for inhibiting chemicals, such as DTT
• No transfer steps – yield is maximized and opportunities for mistakes and
• contamination are minimized
• High DNA recovery – Minimal loss of nucleic acids during extraction
• No magnetic beads – No spin-columns
• Flexibility – suitable for low-throughput to high-throughput extraction with a
• single protocol
• Easily automated using standard liquid handling solutions
• Minimal plasticware required – Reduced waste and supply chain issues
• forensicGEM Sperm is an excellent tool for Y-screening of sexual assault samples prior to differential extraction.