PhytoGEM Plant DNA extraction kit

 

Description

Eliminate the challenges of plant and fungal DNA extraction with phytoGEM, the specialized kit designed for the automated, high-quality isolation of DNA from even the toughest samples. From resilient leaf tissue to complex fungal pathogens, our enzymatic lysis method overcomes inhibitory compounds and tough cell walls, delivering PCR and qPCR-ready DNA without laborious grinding, columns, or hazardous solvents.

 

Designed for Diverse Botanical and Mycological Samples:

Effortlessly extract DNA from a wide variety of plant materials, including leaf, stem, and root tissues, as well as from plant pathogens, fungi, and oomycetes. This makes phytoGEM the ideal tool for studying plant genotypes, genetic variation, endophytic pathogens, genetic mapping, fingerprinting, microsatellite marker-assisted selection, and for verifying the authenticity of botanical extracts.

Overcomes Traditional Extraction Challenges:

Conventional methods struggle with the polysaccharides, tannins, and polyphenols released from plant and fungal cells, requiring multi-step, time-intensive processes that often degrade DNA. phytoGEM’s proprietary, temperature-controlled enzymatic lysis effectively breaks down tough cell walls in a closed-tube system. This gentle approach eliminates harsh chemical washes, preserves DNA integrity, and yields inhibitor-free DNA suitable for sensitive downstream applications.

 

A Simple, Automated Workflow for Rapid Pathogen Detection:

Early detection is critical for crop management, as plant diseases cause significant global agricultural losses. phytoGEM, used with the automated PDQeX Nucleic Acid Extractor, transforms this bottleneck into a rapid, hands-off process. From a simple leaf punch on a storage card or homogenized tissue, the system performs precise, closed-tube lysis and purification, drastically reducing hands-on time and contamination risk. This enables proactive disease control through the rapid detection of critical pathogens.

Validated for Key Agricultural Threats:

The phytoGEM method has been specifically validated for detecting a range of economically significant pathogens, including:

 

• Bacteria: Xylella fastidiosa, Pseudomonas syringae (Psa), Candidatus Liberibacter solanacearum.
• Oomycetes: Phytophthora species.
• Phytoplasma: In grapevines.
• Viruses: Cassava Mosaic Virus.
• Fungi: Providing high-quality DNA for genomics, strain characterization, and environmental studies.

 

Why Researchers Choose phytoGEM:

 

• Breaks Down Tough Cells: Enzymatic lysis efficiently handles plant and fungal cell walls without mechanical grinding.
• Eliminates Inhibitors: Produces clean DNA free from polysaccharides and polyphenols that inhibit PCR.
• Automated & Hands-Off: Enables simple, closed-tube processing on the PDQeX system for high consistency and safety.
• Rapid Results: Accelerates pathogen detection and genetic analysis from hours to minutes.
• Broadly Validated: Trusted for key plant diseases and fungal genomics.

Choose phytoGEM. It is the robust, automated solution for modern plant science and mycology, turning complex extraction into a simple, reliable step towards discovery and crop protection.

 

Application

 

Plant samples including:

 

• leaf
• stem
• root
• plant pathogens
• fungi

 

Kit components

 

• phytoGEM
• Histosolv
• GREEN+ Buffer
• Enhancer