Extraction Tubes

Simplify and accelerate your most demanding DNA extractions with PDQeX Extraction Tubes. These innovative, single-use cartridges are the core of the revolutionary PDQeX system, designed to purify high-quality DNA from challenging samples—including tough plant tissues, forensic swabs, and bacterial cultures—in under 20 minutes. By integrating lysis, purification, and inhibitor removal into a single closed tube, they eliminate manual transfer steps, drastically reduce contamination risk, and deliver PCR or NGS-ready DNA with unprecedented ease.

 

Engineered for Automation

While specifically designed for optimal, hands-free processing in the PDQeX Nucleic Acid Extractor, the cartridges are also compatible with other programmable heat cyclers. This flexibility allows you to integrate streamlined, single-tube chemistry into your existing lab setup.

 

How They Work: A Self-Contained Microfluidic Lab

Each tube is a complete extraction lab. The process is entirely driven by temperature within the sealed cartridge:

• Enzyme-Powered Lysis: A pre-loaded cocktail of thermophilic proteinase and mesophilic cell wall-degrading enzymes efficiently breaks down cells and digests nucleases and proteins upon heating.
• Pressure-Driven Purification: The heating step activates the enzymes and simultaneously increases pressure inside the tube. This forces the lysate through a heat-burstable valve.
• Integrated Cleanup: The sample passes through a proprietary purification matrix that captures cellular debris, inhibitory polyphenols, polysaccharides, and other contaminants.
• Pure Elution: The process yields clean, high-integrity DNA—either double or single-stranded—ready for immediate use in the collection chamber, with no manual intervention required.

 

Key Features & Benefits

Complete Single-Tube Workflow: All steps from sample input to pure DNA output occur in one sealed, disposable cartridge. There are no transfers, spins, or columns.

Exceptional Speed: Achieve PCR-ready DNA in minutes, not hours, dramatically accelerating your time-to-result.

Superior Sample Integrity: The gentle, enzymatic lysis and closed-tube environment protect DNA from degradation and shear.

Effective Inhibitor Removal: The integrated matrix specifically removes common inhibitors from plants, forensic samples, and microbes, ensuring success in sensitive downstream assays.

Enhanced Safety & Consistency: The closed system minimizes aerosol risks and user exposure to samples while providing highly reproducible results.

Flexible Platform Use: Optimized for the PDQeX instrument, with compatibility for other programmable thermal cyclers.

 

Ideal Applications

• Plant & Fungal Genomics: Efficiently extract DNA from leaves, stems, roots, and fungal cultures.
• Forensic Analysis: Process buccal swabs, blood stains, semen samples, and touch DNA.
• Microbiology: Prepare bacterial DNA from cultures or complex backgrounds.
• Next-Generation Sequencing (NGS) & qPCR: Produce the high-purity, inhibitor-free DNA required for these sensitive applications.

 

PDQeX Extraction Tubes are not just consumables; they are the essential, smart component that makes fully automated, reliable nucleic acid extraction a reality for any lab.