Temperature-controlled, hands-free, single-tube processing makes Exymes prepGEM Universal perfectly suited for extracting DNA from cell culture, even single cells, and ensures the integrity of the sample with no loss of DNA.

PrepGEM is an excellent tool for researchers performing confirmatory genotyping of transfected cells following CRISPR/Cas9 gene editing.

For tissue culture, prepGEM Universal gives linear results from 200,000 cells down to 1 single cell. The beauty of the method is that it can be scaled down to sub-µl volumes making it perfect for single-cell work. The kit produces single-stranded DNA which can be used for STR, PCR, WGA and qPCR. The single-step, closed-tube lysis and extraction are highly desirable because MicroGEM extraction buffers are compatible with downstream reactions.

Effortless DNA extraction

PrepGEM Universal utilizes Exymes thermophilic proteinase for simple, rapid DNA extraction with unprecedented recovery.

Sample types: cell culture, Saliva, tissue, blood and insects

Key advantages:

• Single-tube extraction
• Protocols ranging from 4 to 18 minutes
• High DNA Recovery – Minimal loss of DNA during extraction
• No magnetic beads – No spin-columns – No harsh chemicals
• Protocols scalable from 1 cell to millions of cells
• Easily automated on a standard laboratory thermocycler or robotic platform
• Minimal plasticware required – Reduced waste

This kit is an excellent tool for researchers performing confirmatory genotyping of transfected cells following CRISPR/Cas9 gene editing.

Each kit contains: prepGEM, Histosolv, BLUE Buffer, RED+ Buffer, ORANGE+ Buffer