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Temperature-controlled, hands-free, single-tube processing makes prepGEM Universal perfectly suited for extracting DNA from cell culture, even single cells, and ensures the integrity of the sample with no loss of DNA.

prepGEM is an excellent tool for researchers performing confirmatory genotyping of transfected cells following CRISPR/Cas9 gene editing.

Depending on your laboratory equipment and end-use application, MicroGEM offers two options:

1. Using Your Lab Equipment

For tissue culture, prepGEM Universal gives linear results from 200,000 cells down to 1 single cell. The beauty of the method is that it can be scaled down to sub-µl volumes making it perfect for single-cell work.

The kit produces single-stranded DNA which can be used for STR, PCR, WGA and qPCR.

The single-step, closed-tube lysis and extraction are highly desirable because MicroGEM extraction buffers are compatible with downstream reactions.

 

Effortless DNA extraction

 

prepGEM Universal utilizes MicroGEM’s thermophilic proteinase for simple, rapid DNA extraction with unprecedented recovery.

Sample types: cell culture, saliva, tissue, blood, and insects

 

Key advantages:

 

• Single-tube extraction
• Protocols ranging from 4 to 18 minutes
• High DNA Recovery – Minimal loss of DNA during extraction
• No magnetic beads – No spin-columns – No harsh chemicals
• Protocols scalable from 1 cell to millions of cells
• Easily automated on a standard laboratory thermocycler or robotic platform
• Minimal plasticware required – Reduced waste

 

This kit is an excellent tool for researchers performing confirmatory genotyping of transfected cells following CRISPR/Cas9 gene editing.

 

Each kit contains: prepGEM, Histosolv, BLUE Buffer, RED+ Buffer, ORANGE+ Buffer

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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Application note: CRISPR

Application note: Fast and efficient DNA extractions for CRISPR

2. MicroGEM's PDQeX Nucleic Acid Extractor

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Customer Testimonial:-

"Got rid of weeks of anxious wait, use of expensive reagents and plastic ware in CRISPR monoclones screen just with the palm sized MicroGem kit that arrived in our laboratory early this year.  Good quality genomic DNA for PCR amplifications and genotyping from as less as 5000 cells reduces weeks of laboratory work to just few hours. MicroGem is a highly recommended time saving and environment friendly kit and in fact a must have in CRISPR labs - Genome Engineering laboratory, University of Westminster, London, United Kingdom." April 2021

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