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prepGEM Bacteria effortlessly extracts DNA from a wide variety of microbial samples including:
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gram-positive bacteria
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gram-negative bacteria
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protozoa
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colonies and liquid cultures
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biofilm and mucosal
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archaea
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swabs
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metagenomic DNA
(soil, stool, and water)
Depending on your laboratory equipment and end-use application, MicroGEM offers two options:

To use your lab’s existing thermocycler or liquid handler, choose the prepGEM Bacteria Kit, optimized to extract DNA from a broad range of bacterial sample types. The reagents are rigorously tested and QC’ed to guarantee them to be DNA-free.
This kit produces single-stranded DNA suitable for PCR, qPCR, targeted sequencing (amplicon sequencing) from metagenomic samples, and 16S ribosomal DNA sequencing.
Effortless, DNA extraction from a wide range of bacterial samples… in minutes not hours.
MicroGEM’s prepGEM Bacteria kit provides a simple automated approach to studying a wide range of microbial cultures. Every aspect of the MicroGEM method ensures the samples are processed quickly and gently. Minimal sample prep, closed tube processing, and gentle lysis ensure that cross contamination and DNA fragmentation are drastically reduced, making MicroGEM’s extraction process streamlined while preserving the integrity of low abundance
species.
Sample types: colonies, biofilms, liquid cultures, sputum, swabs, soil & stool
Downstream applications: PCR, qPCR, Amplicon Sequencing
Key advantages:
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Single-tube extraction
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Minimal transfer steps - opportunities for mistakes and contamination are
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minimized
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Improved community representation in metagenomic samples
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No ionic detergents or chaotropic salts
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No magnetic beads – No spin-columns
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Flexibility - suitable for low-throughput to high-throughput extraction with a
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single protocol
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Easily automated using standard liquid handling solutions
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Minimal plasticware required - Reduced waste and supply chain issues

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2. Using MicroGEM's PDQeX Nucleic Acid Extractor

MicroGEM’s automated technology provides easy sample preparation and extractions, with added capabilities to generate purified, double-stranded DNA. Use our PDQeX instrument if you are working with inhibitory samples (for example soil and stool) or performing NGS (Next Gen Sequencing) or require double-stranded DNA for library construction.
This kit produces double-stranded DNA suitable for whole genome sequencing, PCR, qPCR, targeted sequencing (amplicon sequencing) from metagenomic samples, and 16S ribosomal DNA sequencing.
Customer Testimonial:-
"I've used the prepGEM Bacteria kits to prepare DNA for microbiome analyses using both high-throughput sequencing and qPCR methods. The DNA is of excellent quality, and the method is quick and easy, minimises contamination risk and represents excellent value for money". Dept of Biosciences, University of Swansea, UK
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